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3D Microscope Imaging System based on the Transport of Intensity Equation

Tianqi Hu
pp.134-140 DOI: 10.62051/vnxzjq61 December 25, 2025
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Abstract: This study aims to develop and implement a low-cost, high-resolution three-dimensional biological sample imaging system based on the light intensity transmission equation (TIE) technology to obtain the three-dimensional phase information of biological samples in a non-destructive way. The system can provide non-staining, real-time three-dimensional reconstruction images through a common optical path design, combined with a standard LED light source, CMOS sensor and optical components. The goal of this study is to provide a simpler and lower-cost imaging technology for biology, medicine and other related fields, especially for cell imaging, pathological analysis and other aspects.
Keywords: TIE; microscopy; 3D imaging.
APA Citation: Tianqi Hu (2025). 3D Microscope Imaging System based on the Transport of Intensity Equation. Transactions on Computer Science and Intelligent Systems Research, 11(1), 134-140. https://doi.org/10.62051/vnxzjq61

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